Diffuse midline glioma (DMG) is a highly aggressive and fatal pediatric high-grade glioma that primarily affects critical regions of the central nervous system, such as the pons, thalamus, and spinal ...

The introduction of real-time quantitative PCR (qPCR) in the early 1990s opened the door for quantitative gene expression analysis. Still, qPCR depends on standard curves, allowing only relative ...